中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (49): 8020-8025.doi: 10.3969/j.issn.2095-4344.2014.49.026

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

力竭运动后24 h模型大鼠肾脏结构和炎症因子对黑加仑提取物干预的反应

刘 霞1，蒋嘉烨2，陆海英3，顾翠英1，蔡美琴4   

  1. 上海中医药大学，1公共健康学院，2教学实验中心，3护理学院，上海市 201203；4上海交通大学医学院，上海市 200025
  • 修回日期:2014-10-20 出版日期:2014-11-30 发布日期:2014-11-30
  • 通讯作者: 蔡美琴，教授，上海交通大学医学院，上海市 200025
  • 作者简介:刘霞，女，1981年生，江苏省盐城市人，硕士，讲师，主要从事运动营养与健康方面的研究。
  • 基金资助:

    上海市教委预算内项目(A1-20140315)

Response of the structure of rat kidney and inflammatory factors to Blackcurrant Extract at 24 hours after exhaustive exercise   

Liu Xia1, Jiang Jia-ye2, Lu Hai-ying3, Gu Cui-ying1, Cai Mei-qin4   

  1. 1School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 3School of Nursing, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 4School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
  • Revised:2014-10-20 Online:2014-11-30 Published:2014-11-30
  • Contact: Cai Mei-qin, Professor, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
  • About author:Liu Xia, Master, Lecturer, School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Supported by:

    the Budget Program of Shanghai Municipal Education Commission, No. A1-20140315

PDF

580

被引次数

2

摘要：

背景：急性运动后肾脏组织易出现运动性肾缺血再灌注现象，自由基生成增加，发生炎症反应，导致肾功能异常。

目的：观察黑加仑提取物对力竭运动后24 h大鼠肾脏结构、肿瘤坏死因子α和核因子κB表达的影响。
方法：30只雄性Wistar健康大鼠随机分为3组，每组10只，黑加仑提取物组给予0.44 g/kg黑加仑提取物灌胃，安静对照组、力竭运动后24 h组灌服同等体积的蒸馏水，连续灌胃6周。力竭运动后24 h组和黑加仑提取物组于末次灌胃后进行无负重游泳运动直至力竭疲劳，于24 h后取材，电镜和光镜观察肾脏组织形态和超微结构变化，免疫组化法检测肿瘤坏死因子α蛋白和核因子κB蛋白表达，RT-PCR检测肿瘤坏死因子α mRNA和核因子κB mRNA的表达。

结果与结论：与安静对照组比较，力竭运动后24 h组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白高表达，核因子κB mRNA和肿瘤坏死因子α mRNA表达水平极显著增加(P < 0.01)；与力竭运动后24 h组比较，黑加仑提取物组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白低表达，核因子κB mRNA表达水平显著性降低(P < 0.05)；肿瘤坏死因子α mRNA表达水平极显著性下降(P < 0.01)。力竭运动后24 h组大鼠肾脏发生明显的形态变化和超微结构损伤，黑加仑提取物组大鼠肾脏结构趋于正常。结果表明黑加仑提取物可修复力竭运动后24 h肾脏组织结构，减少炎症因子的表达，防止肾脏发生炎症性损伤。



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

关键词: 实验动物, 组织工程, 黑加仑提取物, 力竭运动后24 h, 核因子κB, 肿瘤坏死因子α, 肾脏结构

Abstract:

BACKGROUND: The kidney tissues easily affected exercise ischemia reperfusion, increased free radicals and inflammation, resulted in abnormal renal function after acute exercise.

OBJECTIVE: To observe the influence of Blackcurrant Extract on the structure of kidney and expression of tumor necrosis factor-α and nuclear factor-κB at 24 hours after exhaustive exercise.
METHODS: A total of 30 male Wistar rats were randomly divided into three groups (n=10). Rats in the Blackcurrant Extract group were intragastrically administered 0.44 g/kg Blackcurrant Extract. Rats in the quietness control group and 24-hour exhaustive exercise group were intragastrically given an equal volume of distilled water for 6 consecutive weeks. Rats in the 24-hour exhaustive exercise group and Blackcurrant Extract group received no swimming motion until exhaustion fatigue after final intragastric administration. Twenty-four hours later, samples were obtained. Kidney tissue morphology and ultrastructure were observed by electron microscopy and light microscopy. Protein expression of tumor necrosis factor-α and nuclear factor-κB was detected using immunohistochemistry. Tumor necrosis factor-α mRNA and nuclear factor-κB mRNA expression was detected using RT-PCR.
RESULTS AND CONCLUSION: Compared with the quietness control group, tumor necrosis factor-α protein and nuclear factor-κB protein expression in the kidney was higher in the 24-hour exhaustive exercise group, and tumor necrosis factor-α mRNA and nuclear factor-κB mRNA expression was significantly increased (P < 0.01). Compared with the 24-hour exhaustive exercise group, tumor necrosis factor-α protein and nuclear factor-κB protein expression was lower in the Blackcurrant Extract group, and nuclear factor-κB mRNA expression was significantly decreased (P < 0.05); tumor necrosis factor-α mRNA expression was significantly reduced (P < 0.01). Kidney in the 24-hour exhaustive exercise group showed obvious morphological changes and ultrastructural damage. The structure of the kidney in the Blackcurrant Extract group tended to be normal. Results suggested that Blackcurrant Extract can repair the kidney tissue injury, reduce the expression of inflammatory factors, and prevent inflammatory damage in the kidney at 24 hours after exhaustive exercise.


中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: ribes, exercise, kidney, NF-kappa B, tumor necrosis factor-alpha

中图分类号: